Brentford conceded a goal in each half as they were beaten 2-0 by Yeovil Town at Huish Park.
After an even opening Brentford had gained the upper hand but Yeovil captain Paul Huntington headed a goal against the run of play.
And in the second half when The Bees looked set to find a way back into it, Andrew Williams stooped to double the lead.
Brentford could find no way back in to the game and headed back to London with nothing to show for their efforts.
Andrew Welsh had an early header saved by Richard Lee but the game took 15 to 20 minutes to settle down.
There were few openings in the first quarter of the match as both teams were engaged in a struggle to gain the ascendency, particularly in a congested midfield.
Stephen Wright blocked a Dean Bowditch shot on a Yeovil foray forward but Brentford got on top.
They could have taken the lead when a corner fell to Myles Weston 15 yards out but his shot was deflected wide.
Gary Alexander headed wide and Marc Laird could have marked his Brentford début with a goal when he arrived to meet a Marcus Bean knock down but his shot was saved by Stephen Henderson low to his left.
The opening goal came against the run of play when Yeovil were awarded a dubious free kick, Welsh curled it in and Huntington beat Lee to the high ball to head home.
Brentford continued to have the better of possession and territory despite the goal.
Leon Legge headed wide and Alexander had a shot blocked before the break and the game game continued in the same vein after the re-start.
Alexander headed wide from close range and then saw a shot smothered by Henderson before Bean headed a Kevin O'Connor corner wide.
The start of the second half was played almost exclusively in the Yeovil penalty area.
On more than one occasion did the ball pinball around the box before the home side scrambled it away.
Brentford thought they should have had a penalty when Charlie MacDonald got in behind the defence after a quick Wright throw in and seemed to get pulled down by Max Ehmer but referee Andy Woolmer waved away the appeals.
And against the run of play, Yeovil doubled their lead.
Bowditch got away on the left and fed the overlapping Nathan Smith, Andrew Williams stooped to head the cross in from close range and give Yeovil a match-winning lead.
The home side had been pushed back for long periods but grew in confidence after the second goal.
Bowditch curled an effort inches wide after a good run and Lee made a great save when Andrew Williams broke down the left and fired towards the far corner.
Yeovil soon reverted to type and sat back, inviting Brentford to come at them and breaking quickly.
The home defence - well marshalled by Huntington - was too tough an obstacle for The Bees and although they pressed there was no way through.
Yeovil almost added a third in the dying seconds when he curled a free kick against the crossbar but it proved not to matter for the home side.
Yeovil Town: Henderson; Ehmer, Ayling, Huntington, Smith; Welsh, Upson, Wotton, Bowditch (sub Gibson 90 mins); S. Williams; A. Williams
Subs (not used): Martin, Jones, Calver, Virgo, Baggridge
Bookings: S. Williams (71 mins), Smith (90 mins)
Brentford: Lee; Wright, Legge (sub Balkestein 72 mins), Osborne, Woodman; Bean (sub Forster 60 mins), Laird, O'Connor, Weston; Alexander (sub Simpson 67 mins), MacDonald
Subs (not used): Royce, McCracken, Spillane, Diagouraga
Bookings: Woodman (15 mins) (fourth of season), Forster (71 mins), Wright (80 mins) (second of season)
By Chris Wickham